Sub-Saharan Africa (SSA) is undergoing rapid urbanization and dependence on groundwater is expected to increase, especially for the urban poor. Accurate quantification of groundwater use is necessary to develop measures to protect and enhance the resource. The current scheme of global water access monitoring, done by the Joint-Monitoring Programme (JMP), neglects groundwater use when offered as piped water and also neglects self-supply of groundwater when in use as a secondary source. The present study aims to quantify urban groundwater use for Uganda and compare it with JMP national data. A method will be developed to estimate urban groundwater use through Geographic Information Systems by coupling the spatially distributed urban water cycle, urban structure analysis, and high resolution population density. The calculated groundwater use is expected to show that current JMP data underestimates the proportion of population using groundwater. The method developed in this research will be applicable to other cities in the SSA region, and provides a tool for further understanding groundwater dependence and supports evidence-based groundwater management measures.