This study deals with the spring waters flow in münsterland in west of Germany in the area extended between Baumberge and Schöppinger berg, which represented closed groundwater system and fractured pore system. Eight springs, four in Baumberge (Arning ost, Aring west, Stever a, Stever new), and four in Schöppinger berg (Schwarthof, Wearning, Leerbach and Kirche ). The springs have been addressed for the purpose of water evaluation for hydrogeology and ecology. In order to meet the objectives of this research, all springs were sampled and analyzed three time in November 2018, January and April 2019 for field parameters (the electrical conductivity, pH value , temperature, dissolved oxygen concentration, While for two time November 2018, and April 2019 for major cations (Mg2+, Ca2+, K+, Na+, Al +, Sr2+), major anions (SO42-, Cl-, HCO3- ) minor anions (PO43-and NO3-) as well as the heavy elements that included (Pb, Zn, Cd, Ni, Fe, Mn, Cu, Cr) and total organic carbon. In addition to isotopes δ34S and biological analysis such as biomass, activity, furthermore faunal measurements for example body size (macrofauna and meiofauna), the ratio of stygobiont to stygoxene fauna and the number of stygofauna per groundwater filled aquifer volume.The organisms were separated from sediments, counted and pre-sorted into taxonomic groups (crustaceans, oligochaets, water mites, nematodes, turballarians). All crustacean groups, which constitute the major part of the animals in groundwater samples, and oligochaets were determined to species level, whilst the remaining taxonomic groups were identified to order or higher level. The results revealed that the springs belong to the classified as Ca-HCO3, which show that most the same hydrochemical properties and only slight variations were observed. Compared to priveous study , there are no major changes in hydrochemistry. However, the evolution of nitrate concentrations in groundwater, which has been described shows a tendency to increase NO3 Concentration. In contract the ecological result refer to determination of the groundwater animals, more from 7 groups of individuals were identified and a total of 642 Copepoda Cyclopoida, 14 Copepoda Harpacticoida, 23 Ostracoda, 17 Nematoda and estimated 162 Nauplius larvae could be detected over the sampling period. The determination show the Arning west and Leerbach largest biodiversity, which is probably due to the high food supply. At the sources. At Kirche no more species were detected. The reason for this could be an influence on the settlement or lack of detritus as a food source from the development in addition to defecult fauna sampling in this spring.