Constructed wetlands have become widespread for pesticide mitigation. Fate and transport of reactive compounds inside these systems involves complex physical, chemical and biological processes that are still not fully understood. Hydrological tracers have been proven to be cost-effective tools to investigate pesticide transport and associated risks in these environments. However, most studies have considered constructed wetlands as "black box" systems. Here we present a novel approach that combines the use of hydrological tracers and high vertical-resolution sampling and monitoring to evaluate pesticide transport and dissipation processes within a wetland system on a long term basis and detailed spatial scale. Three tracers with different sorptive and reactive properties (bromide (Br), uranine (UR) and sulforhodamine B (SRB)) were applied together with three selected pesticides (boscalid, penconazole and metazachlor). The influence of vegetation and alternating different hydrologic conditions on pesticide transport and dissipation were evaluated by comparing a vegetated with a non-vegetated section of the system and by alternating periods of saturation and drying, respectively. Breakthrough curves obtained at different sampling depths revealed that the solutes were not equally distributed within the constructed wetland. Pre-injection conditions, i.e., system at field capacity, probably caused heterogeneities as a result of the existence of water-filled pores in the areas adjacent to the sampling ports of the sandy layers, especially in the middle sections. This was evidenced by a delay in the arrival of the breakthrough peaks. Data also revealed that a higher mass of solutes was transported to the vegetated part of the uppermost layer. We hypothesized that the plant roots could have acted as a shortcut. The strong temporal and spatial correlation found between Br, UR and metazachlor suggested that these solutes experienced greater transport than SRB, boscalid and penconazole, which judging by their rapid decrease in their concentration mostly underwent sorption. This was later confirmed by their similar gradual increase in accumulated mass recovery at the outlet during the flushing phase. Biochemical transformation contributed to the dissipation of metazachlor, as evidenced by the measurement of its transformation products, whose peaks were detected when the aerobic conditions were promoted. This study represents a first approximation to the joint use of hydrological tracers and high vertical-resolution sampling and monitoring to study pesticide behavior inside constructed wetlands with great spatial and temporal detail. Further experiments need to be done under field conditions coupled with mathematical modeling to provide additional insights into the complex phenomena related to transport and dissipation of pollutants in wetland systems.